Antibody used in a multicolor flow cytometry panel to phenotype PBMCs and expanded CAR T cells. Works very well in separation with CD8 (BD Horizon #566860). 200-300.000 PBMCs or T cells were washed once in PBS and stained in approximately 100 uL PBS + 2 % FCS using 1 uL antibody (antibody has been titrated on T cells).
On June 10, 2020 Kasper Mølgaard Jensen wrote:
Antibody used in a multicolor flow cytometry panel to phenotype PBMCs and expanded CAR T cells. Works very well in separation with CD8 (BD Horizon #566860). 200-300.000 PBMCs or T cells were washed once in PBS and stained in approximately 100 uL PBS + 2 % FCS using 1 uL antibody (antibody has been titrated on T cells).
On September 29, 2018 Arianna Draghi, Center for Cancer Immune Therapy, Herlev Hospital wrote:
CD4 (human), mouse mAb (clone SK3), BV510 conjugated, BD Biosciences, 562970
Used to stain lymphocytes in an activation panel along with (CD8 and CD137).
2uL used to stain cells in a FACS tube at 4 degrees.