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On May 24, 2021 Acaimo G-R wrote:
Rabbit polyclonal anti-Perlecan. A 2310bp cDNA coding for Domain V of the Perlecan protein was codon optimised for its expression in mammalian cells and ligated into an episomal expression vector. The vector was transfected into human 293-EBNA cells and serum- free medium was collected for protein purification. Antibodies were obtained after Ni-affinity purification followed by size-exclusion chromatography. Immunisation of rabbits and affinity-purification of antibodies followed standard protocols. The affinity-purified antibody was used at a concentration of 1:2000.
On June 17, 2020 PKM2, Rabbit mAb, Cell Signaling Technology, Cat#4053S. wrote:
Used on BMDC:
for Western blot at the dilution of 1:1000;
for IF at the dilution of 1:200;
for IP at the dilution of 1:50;
On April 13, 2020 Ferenc Livak wrote:
Rabbit monoclonal anti-RAG2 (mAb 39) used for CHIP_Seq at 10 ug/mL final concentration. Intermediate performance. Antibody is not available commercially.
On April 13, 2020 Ferenc Livak wrote:
Rabbit monoclonal anti-RAG1 (mAb 23)used for CHIP_Seq at 10 ug/mL final concentration. Intermediate performance. Antibody is not available commercially.
On March 2, 2018 Pall Karlsson wrote:
Rabbit dopamine beta hydroxylase (DbH) antibody, used in immunostaining (fluorescence).
Concentration: 1:150
Manufacturer: Chemicon/Millipore
Product ID: B-1065
Works wonderfully to visualize arrector pili innervation/pilomotor nerve fibers in human skin tissue (cut 50 micrometer thick cryosections). Antibody left on over-night on shaker table and secondary antibody for 2 hours.
On March 1, 2018 Marianne Lundsgaard Kristensen wrote:
Collagen IV – rabbit anti-human from Biodesign
Used on eyes from mice to stain lens capsule.
Concentration 1:100
On May 13, 2017 Sally Carter wrote:
This antibody does not work at all for Western blot.
On November 11, 2015 Alice Vickers wrote:
Vimentin rabbit polyclonal antibody, St John’s Laboratory (STJ31262).
Tested for western blot, dilution 1:1000.
The antibody was specific and the image captured was clear.
On November 11, 2015 Alice Vickers wrote:
Tubulin beta rabbit polyclonal antibody, St John’s Laboratory (STJ31562).
Tested on U251 and U373 glioblastoma for immunocytochemistry and immunofluorescence. Primary antibody was diluted 1:200.
Staining was positive for cytoplasmic, vesicle-like structures.
On November 11, 2015 Alice Vickers wrote:
RARA rabbit polyclonal antibody, St John’s Laboratory (STJ26160).
Tested tested in western blotting on subventricular zone neural stem cells isolated from mice (primary culture). Primary antibody was diluted 1:1000 in TBS containing 0.1% porcine gelatin.
The antibody tested gave a clear band at the predicted molecular weight.
On November 11, 2015 Alice Vickers wrote:
PIWIL4 rabbit polyclonal antibody, St John’s Laboratory (STJ25009).
Tested on protein extracts from rat testis for western blot, dilution 1:1000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
PCNA rabbit polyclonal antibody, St John’s Laboratory (STJ94982).
Tested on 10µg protein extract from HeLa and Jurkat cell lines for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:10,000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
p53 rabbit polyclonal antibody, St John’s Laboratory (STJ94890).
Tested on 10µg protein extract from Jurkat cell lysates for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:10,000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
NFκB-p65 rabbit polyclonal antibody, St John’s Laboratory (STJ94468).
Tested on 10µg protein extract from HeLa cell lysates for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:10,000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
NCKX4 rabbit polyclonal antibody, St John’s Laboratory (STJ94359).
Tested on HEK/MEB4/MNT-1 cells for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:2500.
The results were positive and the antibody will be used for further work.
On November 11, 2015 Alice Vickers wrote:
NCKX3 rabbit polyclonal antibody, St John’s Laboratory (STJ94358).
Tested on HEK/MEB4/MNT-1 cells for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:2500.
The results were positive and the antibody will be used for further work.
On November 11, 2015 Alice Vickers wrote:
NANOG mouse monoclonal antibody, St John’s Laboratory (STJ31850).
Used for immunohistochemistry, dilution 1:500.
The antibody gave conclusive results.
On November 11, 2015 Alice Vickers wrote:
mTOR rabbit polyclonal antibody, St John’s Laboratory (STJ94280).
Tested on 10µg protein extract from HeLa and Jurkat cell lines for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:10,000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
mGluR8 rabbit polyclonal antibody, St John’s Laboratory (STJ94114).
Tested on rat primary microglial cells for immunohistochemistry and immunofluorescence, dilution 1:200.
The antibody gave conclusive results.
On November 11, 2015 Alice Vickers wrote:
mCherry tag mouse monoclonal antibody, St John’s Laboratory (STJ34373).
Tested on A549 cells transfected with mCherry vector pLV-mCherry (positive control) and untransfected A549 cells (negative control) for immunofluorescence. Primary antibody was diluted 1:200. Secondary antibody (goat anti-mouse IgG+IgM Antibody (DyLight™ 488))was diluted 1:200.
Specific fluorescent signal was detected in the positive control, and no signal was detected in the negative control.
On November 11, 2015 Alice Vickers wrote:
LPCAT1 rabbit polyclonal antibody, St John’s Laboratory (STJ27005).
Tested on protein extract from mouse lung tissue for western blot, dilution 1:1000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
HA-tag rabbit polyclonal antibody, St John’s Laboratory (STJ90107).
Tested on protein extracts from HeLa and Jurkat cell lysates for western blot. Primary antibody was diluted 1:1000. Secondary antibody (HRP goat anti-rabbit) was diluted 1:10,000.
The target band was found in the material cell lines.
On November 11, 2015 Alice Vickers wrote:
GSK3β rabbit polyclonal antibody, St John’s Laboratory (STJ93447).
Tested on 10µg protein extract from HeLa cell lysates for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:10,000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
GAPDH rabbit polyclonal antibody, St John’s Laboratory (STJ96417).
Tested on 10µg protein extract from HeLa and Jurkat cell lines for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:10,000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
FAAH rabbit polyclonal antibody, St John’s Laboratory (STJ23602).
Tested on dorsal root ganglia for immunofluorescence, dilution 1:50.
The antibody stained very well.
On November 11, 2015 Alice Vickers wrote:
ERBB4 rabbit polyclonal antibody, St John’s Laboratory (STJ27886).
Tested on 15µg protein extract from MCF7 cells for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:2500.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
EphB4 rabbit polyclonal antibody, St John’s Laboratory (STJ23550).
Tested on 15µg protein extract from MCF7 cells for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:2500.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
EphA2 rabbit polyclonal antibody, St John’s Laboratory (STJ92940).
Tested on 10µg protein extract from MDA-MB-231 cells for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:2500.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
ELMOD2 rabbit polyclonal antibody, St John’s Laboratory (STJ27186).
Tested on 10ng GST-ELMOD2 purified recombinant fusion protein and 10µg protein extract from HeLa cell lysates for western blot. Primary antibody was diluted 1:500.
The antibody works in immunoblots and can detect low levels (ng) of protein.
On November 11, 2015 Alice Vickers wrote:
D2HGDH rabbit polyclonal antibody, St John’s Laboratory (STJ27209).
Tested on frozen sections of mouse muscle for immunofluorescent staining, dilution 1:100.
The antibody worked, with a positive and specific expression of H2HGDH at the neuromuscular junction in the muscle tissue from mice. There was also some absorption of the antibody by muscle fibres.
On November 11, 2015 Alice Vickers wrote:
Cdk2 rabbit polyclonal antibody, St John’s Laboratory (STJ92197).
Tested on 10µg protein extract from HeLa and Jurkat cell lines for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:10,000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
Cdc2 rabbit polyclonal antibody, St John’s Laboratory (STJ92154).
Tested on 10µg protein extract from HeLa and Jurkat cell lines for western blot. Primary antibody was diluted 1:1000. Secondary antibody was diluted 1:10,000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
CD133 rabbit polyclonal antibody, St John’s Laboratory (STJ20168).
Tested on human endothelial progenitor cells isolated from stroke patients for immunocytochemistry. Primary antibody diluted 1:200 in PBS containing 0.1% porcine gelatin.
The antibody tested stained intensely at the membrane as predicted.
On November 11, 2015 Alice Vickers wrote:
CAR rabbit polyclonal antibody, St John’s Laboratory (STJ91996).
Tested on 15µg protein extract from MCF7 cells for western blot. Primary antibody was diluted 1:1000. Secondary antibody (anti-rabbit IgG HRP-linked ) was diluted 1:2500.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
CAMSAP3 rabbit polyclonal antibody, St John’s Laboratory (STJ46575).
Tested on untransfected HUVECs and HUVECs transfected with GFP-CAMSAP3 for western blot. Dilution 1:1000.
There was a clear band at the expected molecular weight (~130kDa).
On November 11, 2015 Alice Vickers wrote:
HA Tag mouse monoclonal antibody, St John’s Laboratory (STJ34368).
Used for western blot, dilution 1:1000.
Target bands of the expected size were detected by the antibody.
On November 11, 2015 Alice Vickers wrote:
Alpha-synuclein rabbit polyclonal antibody, St John’s Laboratory (STJ95862).
Tested on rat substantia nigra fixed-frozen tissue for immunohistochemistry, dilution 1:200.
There was clear staining of endogenous alpha-synuclein protein
On November 11, 2015 Alice Vickers wrote:
Beta-actin rabbit polyclonal antibody, St John’s Laboratory (STJ91464)
Tested on 10µg from HeLa and Jurkat cell lines for Western blot. Primary antibody was diluted 1:1000. Secondary antibody (HRP goat anti-rabbit) was diluted 1:10,000.
Target bands of the expected size were detected by the antibody.