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On November 17, 2023 Kasper Mølgaard Jensen wrote:
Anti-TROP2 APC conjugated antibody from Miltenyi (#130-115-056) was used to stain TROP2 positive SKOV-3, OVCAR3 and PC-3 tumour cell lines (ATCC). PA-1 cells (ATCC) were used as a negative control. Antibody worked well at 1uL in 50uL master (along with a dead stain) in PBS + 2% FBS + 2mM EDTA. Works with fixation in 0.9% formaldehyde and PBS.
On May 8, 2023 Kasper Mølgaard Jensen wrote:
anti-human CD45 PE-conjugated, clone HI30, #304058 (Nordic Biosite). Recommended staining volume is 5 uL in a total of 50 uL staining mix. This antibody was titrated using PBMCs and the optimal staining index (SI) was found at a 1:20 dilution (2.5 uL in 50 uL) in cold PBS supplemented with 2% FBS and 1mM EDTA.