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On August 23, 2022 Priyanka Upadhyai wrote:
21A6, mouse monoclonal, DSHB (AB_528449)
Sample: embryos
Fixation: 4% formaldehyde
Dilution 1:30
Performance: good signal detected at proximal ciliary tips
On August 23, 2022 Priyanka Upadhyai wrote:
HRP, rabbit polyclonal, Jackson Immunoresearch (cat # 323-005-021)
Sample: embryos
Fixation: 4% formaldehyde
Dilution: 1:500
Performance: worked well
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synaptogyrin1, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 110 011
Sample: Rat brain tissue (P14). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:200
Performance: No specific signal observed.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synaptotagmin 5/9, rabbit, polyclonal prim. AB, Synaptic Systems 105 053
Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: In presynaptic region. Good signal.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synaptotagmin 7, rabbit, polyclonal prim. AB, Synaptic Systems 105 173
Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:100
Performance: No signal in MNTB region.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synaptotagmin 3, rabbit, polyclonal prim. AB, Synaptic Systems 105 133
Sample: Rat brain tissue (P12). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Specific signal at presynapse.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synaptophysin, mouse/IgG1, monoclonal prim. AB, Synaptic Systems 101 011
Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Good signal. But substantially less compared to the polyclonal Synaptophysin AB (101 002)
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synaptogyrin1, rabbit, polyclonal prim. AB, Synaptic Systems 103003
Sample: Rat brain tissue (P12). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Specific for presynapse. Very good signal.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synaptogyrin1, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 103 011
Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Very sparse signal. Possibly, not specific. In contrast, the polyclonal Synaptogyrin1 (SySy 103 003) works very well and specific.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synapsin 3, rabbit, polyclonal prim. AB, Synaptic Systems 106 303
Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Very sparse but presumably specific signal at the presynapse.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synapsin 2, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 106 211
Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:200
Performance: Very sparse signal.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synapsin 1, mouse/IgG1, monoclonal prim. AB, Synaptic Systems 106 011 (corrected)
Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:200
Performance: Very sparse signal.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Synapsin 1, mouse/IgG1, monoclonal prim. AB, Synaptic Systems 188 011
Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:200
Performance: Very sparse signal.
On August 2, 2021 MultiNano Group Heidelberg wrote:
SV2a, rabbit, polyclonal prim. AB, Synaptic Systems 119 002
Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Very sparse but presumably specific signal at the presynapse.
On August 2, 2021 MultiNano Group Heidelberg wrote:
SNAP25, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 111 111
Sample: Rat brain tissue (P14). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Specific signal. More signal in close proximity to the presynaptic membranes.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Munc18-1, mouse/IgG2a KLC, monoclonal prim. AB, Synaptic Systems 116 011
Sample: Rat Brain tissue (P13). Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Very sparse signal. Possibly, not specific.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Mint1, rabbit, polyclonal prim. AB, Synaptic Systems 144 103
Sample: Brain tissue. Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Very sparse signal. Possibly, not specific.
On August 2, 2021 MultiNano Group Heidelberg wrote:
MAP2, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 188 011
*
Sample: Brain tissue. Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Not specific. In contrast to the MAP2 AB from chicken (SySy 188 006), that is specific for the postsynaptic principle cell.
On August 2, 2021 MultiNano Group Heidelberg wrote:
Endophilin 1, rabbit, polyclonal prim. AB, Synaptic Systems 159 002
Sample: Brain tissue. Calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Very specific signal in small region of the presynapse.
On August 2, 2021 CSP, rabbit, polyclonal prim. AB, Synaptic Systems 154003 wrote:
Sample: Brain tissue, at the calyx of Held in the medial nucleus of the trapeziod body.
Fixation: 4% PFA perfusion
Dilution: 1:500
Performance: Sparse signal, but seems to be specific.
On August 2, 2021 Piccolo, rabbit pAb, Synaptic Systems, 142 113 wrote:
Used in brain tissue, at the calyx of Held in the medial nucleus of the trapeziod body. Results in a signal specific for the active zone (AZ); verified by colocalization to the AZ marker Bassoon.
Fixation: 4% PFA perfusion
Dilution: 1:500
On July 15, 2021 Simon Jensen wrote:
Rabbit monoclonal anti-RAG1 (mAb 23)used for CHIP_Seq at 10 ug/mL final concentration. Intermediate performance. Antibody is not available commercially.
On May 24, 2021 Acaimo G-R wrote:
Rabbit polyclonal anti-Perlecan. A 2310bp cDNA coding for Domain V of the Perlecan protein was codon optimised for its expression in mammalian cells and ligated into an episomal expression vector. The vector was transfected into human 293-EBNA cells and serum- free medium was collected for protein purification. Antibodies were obtained after Ni-affinity purification followed by size-exclusion chromatography. Immunisation of rabbits and affinity-purification of antibodies followed standard protocols. The affinity-purified antibody was used at a concentration of 1:2000.
On June 17, 2020 PKM2, Rabbit mAb, Cell Signaling Technology, Cat#4053S. wrote:
Used on BMDC:
for Western blot at the dilution of 1:1000;
for IF at the dilution of 1:200;
for IP at the dilution of 1:50;
On April 13, 2020 Ferenc Livak wrote:
Rabbit monoclonal anti-RAG2 (mAb 39) used for CHIP_Seq at 10 ug/mL final concentration. Intermediate performance. Antibody is not available commercially.
On April 13, 2020 Ferenc Livak wrote:
Rabbit monoclonal anti-RAG1 (mAb 23)used for CHIP_Seq at 10 ug/mL final concentration. Intermediate performance. Antibody is not available commercially.
On March 2, 2018 Pall Karlsson wrote:
Rabbit dopamine beta hydroxylase (DbH) antibody, used in immunostaining (fluorescence).
Concentration: 1:150
Manufacturer: Chemicon/Millipore
Product ID: B-1065
Works wonderfully to visualize arrector pili innervation/pilomotor nerve fibers in human skin tissue (cut 50 micrometer thick cryosections). Antibody left on over-night on shaker table and secondary antibody for 2 hours.
On March 1, 2018 Marianne Lundsgaard Kristensen wrote:
Collagen IV – rabbit anti-human from Biodesign
Used on eyes from mice to stain lens capsule.
Concentration 1:100