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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM

28 Pending Reviews

  • 21A6, mouse monoclonal, DSHB (AB_528449)
    Sample: embryos
    Fixation: 4% formaldehyde
    Dilution 1:30
    Performance: good signal detected at proximal ciliary tips

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • HRP, rabbit polyclonal, Jackson Immunoresearch (cat # 323-005-021)
    Sample: embryos
    Fixation: 4% formaldehyde
    Dilution: 1:500
    Performance: worked well

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Synaptogyrin1, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 110 011
    Sample: Rat brain tissue (P14). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:200
    Performance: No specific signal observed.

  • Synaptotagmin 5/9, rabbit, polyclonal prim. AB, Synaptic Systems 105 053
    Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: In presynaptic region. Good signal.

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Synaptotagmin 7, rabbit, polyclonal prim. AB, Synaptic Systems 105 173
    Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:100
    Performance: No signal in MNTB region.

    Western blotNot Rated
    Immunostaining11111
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Synaptotagmin 3, rabbit, polyclonal prim. AB, Synaptic Systems 105 133
    Sample: Rat brain tissue (P12). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Specific signal at presynapse.

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Synaptophysin, mouse/IgG1, monoclonal prim. AB, Synaptic Systems 101 011
    Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Good signal. But substantially less compared to the polyclonal Synaptophysin AB (101 002)

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Synaptogyrin1, rabbit, polyclonal prim. AB, Synaptic Systems 103003
    Sample: Rat brain tissue (P12). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Specific for presynapse. Very good signal.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Synaptogyrin1, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 103 011
    Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Very sparse signal. Possibly, not specific. In contrast, the polyclonal Synaptogyrin1 (SySy 103 003) works very well and specific.

  • Synapsin 3, rabbit, polyclonal prim. AB, Synaptic Systems 106 303
    Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Very sparse but presumably specific signal at the presynapse.

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Synapsin 2, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 106 211
    Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:200
    Performance: Very sparse signal.

    Western blotNot Rated
    Immunostaining22222
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Synapsin 1, mouse/IgG1, monoclonal prim. AB, Synaptic Systems 106 011 (corrected)
    Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:200
    Performance: Very sparse signal.

  • Synapsin 1, mouse/IgG1, monoclonal prim. AB, Synaptic Systems 188 011
    Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:200
    Performance: Very sparse signal.

    Western blotNot Rated
    Immunostaining22222
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • SV2a, rabbit, polyclonal prim. AB, Synaptic Systems 119 002
    Sample: Rat brain tissue (P21). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Very sparse but presumably specific signal at the presynapse.

    Western blotNot Rated
    Immunostaining22222
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • SNAP25, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 111 111
    Sample: Rat brain tissue (P14). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Specific signal. More signal in close proximity to the presynaptic membranes.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Munc18-1, mouse/IgG2a KLC, monoclonal prim. AB, Synaptic Systems 116 011
    Sample: Rat Brain tissue (P13). Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Very sparse signal. Possibly, not specific.

    Western blotNot Rated
    Immunostaining11111
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Mint1, rabbit, polyclonal prim. AB, Synaptic Systems 144 103

    Sample: Brain tissue. Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Very sparse signal. Possibly, not specific.

    Western blotNot Rated
    Immunostaining22222
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • MAP2, mouse/IgG1 KLC, monoclonal prim. AB, Synaptic Systems 188 011
    *
    Sample: Brain tissue. Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Not specific. In contrast to the MAP2 AB from chicken (SySy 188 006), that is specific for the postsynaptic principle cell.

    Western blotNot Rated
    Immunostaining11111
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Endophilin 1, rabbit, polyclonal prim. AB, Synaptic Systems 159 002

    Sample: Brain tissue. Calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Very specific signal in small region of the presynapse.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Sample: Brain tissue, at the calyx of Held in the medial nucleus of the trapeziod body.
    Fixation: 4% PFA perfusion
    Dilution: 1:500
    Performance: Sparse signal, but seems to be specific.

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Used in brain tissue, at the calyx of Held in the medial nucleus of the trapeziod body. Results in a signal specific for the active zone (AZ); verified by colocalization to the AZ marker Bassoon.
    Fixation: 4% PFA perfusion
    Dilution: 1:500

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Rabbit monoclonal anti-RAG1 (mAb 23)used for CHIP_Seq at 10 ug/mL final concentration. Intermediate performance. Antibody is not available commercially.

    Western blot44444
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Rabbit polyclonal anti-Perlecan. A 2310bp cDNA coding for Domain V of the Perlecan protein was codon optimised for its expression in mammalian cells and ligated into an episomal expression vector. The vector was transfected into human 293-EBNA cells and serum- free medium was collected for protein purification. Antibodies were obtained after Ni-affinity purification followed by size-exclusion chromatography. Immunisation of rabbits and affinity-purification of antibodies followed standard protocols. The affinity-purified antibody was used at a concentration of 1:2000.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Used on BMDC:
    for Western blot at the dilution of 1:1000;
    for IF at the dilution of 1:200;
    for IP at the dilution of 1:50;

  • Rabbit monoclonal anti-RAG2 (mAb 39) used for CHIP_Seq at 10 ug/mL final concentration. Intermediate performance. Antibody is not available commercially.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIP33333
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Rabbit monoclonal anti-RAG1 (mAb 23)used for CHIP_Seq at 10 ug/mL final concentration. Intermediate performance. Antibody is not available commercially.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIP33333
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Rabbit dopamine beta hydroxylase (DbH) antibody, used in immunostaining (fluorescence).
    Concentration: 1:150
    Manufacturer: Chemicon/Millipore
    Product ID: B-1065

    Works wonderfully to visualize arrector pili innervation/pilomotor nerve fibers in human skin tissue (cut 50 micrometer thick cryosections). Antibody left on over-night on shaker table and secondary antibody for 2 hours.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Collagen IV – rabbit anti-human from Biodesign
    Used on eyes from mice to stain lens capsule.
    Concentration 1:100

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated