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On May 8, 2023 Kasper Mølgaard Jensen wrote:
anti-human CD45 PE-conjugated, clone HI30, #304058 (Nordic Biosite). Recommended staining volume is 5 uL in a total of 50 uL staining mix. This antibody was titrated using PBMCs and the optimal staining index (SI) was found at a 1:20 dilution (2.5 uL in 50 uL) in cold PBS supplemented with 2% FBS and 1mM EDTA.