I have used it for IHC on mouse DRG (dorsal root ganglia), at a dilution of 1:100, in combination with Fab-fragments, without success! I could not get it to work properly.
On April 11, 2014Thaneas Prabakaran, Aarhus University wrote:
5
It works very well for immunostaining of PFA fixed cells at a dilution of 1:100
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On August 20, 2013Stine Klinger wrote:
0
Not reactive against rat sortilin
On August 6, 2013Camilla Gustafsen wrote:
5
We have used this antibody as detection antibody (1 µg/ml) in a sortilin specific ELISA used for quantification of soluble sortilin in human plasma. As capture antibody we used pAb anti-sortilin/F7615 (10 µg/ml).
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ELISA
Flow cytometry
Not Rated
Luminex
Not Rated
On August 6, 2013Camilla Gustafsen wrote:
5
This is a very good antibody for immunostaining of endogenous sortilin in HepG2 cells. Gives nice staining at concentration 1 μg/ml.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 10, 2012Simon Glerup, Aarhus wrote:
5
Great sortilin antibody for detection in ELISA.
Dilution 0.05 to 1 ug/ml
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ELISA
Flow cytometry
Not Rated
Luminex
Not Rated
On April 14, 2012Anja A, Aarhus wrote:
4
This mAb works for the detection of mouse sortilin by IF in cultured cerebellar granule neurons and by IHC on brain sections.
Dilution 1:100
On October 28, 2014 Laura Johnsen wrote:
I have used it for IHC on mouse DRG (dorsal root ganglia), at a dilution of 1:100, in combination with Fab-fragments, without success! I could not get it to work properly.
On April 11, 2014 Thaneas Prabakaran, Aarhus University wrote:
It works very well for immunostaining of PFA fixed cells at a dilution of 1:100
On August 20, 2013 Stine Klinger wrote:
Not reactive against rat sortilin
On August 6, 2013 Camilla Gustafsen wrote:
We have used this antibody as detection antibody (1 µg/ml) in a sortilin specific ELISA used for quantification of soluble sortilin in human plasma. As capture antibody we used pAb anti-sortilin/F7615 (10 µg/ml).
On August 6, 2013 Camilla Gustafsen wrote:
This is a very good antibody for immunostaining of endogenous sortilin in HepG2 cells. Gives nice staining at concentration 1 μg/ml.
On November 10, 2012 Simon Glerup, Aarhus wrote:
Great sortilin antibody for detection in ELISA.
Dilution 0.05 to 1 ug/ml
On April 14, 2012 Anja A, Aarhus wrote:
This mAb works for the detection of mouse sortilin by IF in cultured cerebellar granule neurons and by IHC on brain sections.
Dilution 1:100