P2X7, rat mAb, clone Hano43, Alexa 488 conjugated, Novus Biologicals, NBP1-40894AF488

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  • monoclonal rat anti-P2X7 conjugated to dylight 488, NBP1-40894AF488, Novusbio

    The antibody was tested in the following dilution series: 1:200; 1:400; 1:800 and 1:1600. Stock concentration: 0.80 mg/mL

    The mouse dorsal root ganglia had beed fixed for 1.5 hrs in PFA. Cryoprotected overnight in 30% sucrose and finally embeded in tissue tek. The sections were cut in 10 um sections.

    The antibody bound unspecific to the DRG neurons (the DRG neurons do not express P2X7 according to the literature). The staining looked the same at all tested concentrations

    Western blotNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Flow cytometry