PPAR gamma (H-100), rabbit pAb, Santa Cruz sc-7196

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  • We tested by western blot a rabbit polyclonal PPARγ Antibody (H-100) cat# sc-7196. It is raised against an epitope corresponding to amino acids 8-106 mapping at the N-terminus of PPARγ1 of human origin. As shown in figure, this antiserum detects (@1:100; 40 microg proteins) multiple bands in brown adipose tissue and brain lysates from the mouse, but no clear band at the expected molecular size.

    Using a standard protocol, the proteins were extracted by homogenizing fat and brain samples in lysis buffer, resolved by SDS-PAGE and then transferred to a nitrocellulose membrane by electroblotting (100 V for 1 to 2 hours). The membrane was incubated with the primary antiserum overnight at 4°C and with the secondary antibody for 1 hour at room temperature.

    Western blot11111
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
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Western blot
Flow cytometry