On April 19, 2016Marie-Louise Holm, Aarhus University wrote:
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This antibody should specifically recognize human ApoE4 and not ApoE2 or ApoE3.
However, the Western blot above shows equal recognition of both ApoE4 and ApoE2.
Lane #1 is medium from astrocytes producing ApoE2.
Lane #2 is medium from astrocytes producing ApoE4.
The intense band is around 38 kDa.
On April 19, 2016 Marie-Louise Holm, Aarhus University wrote:
This antibody should specifically recognize human ApoE4 and not ApoE2 or ApoE3.
However, the Western blot above shows equal recognition of both ApoE4 and ApoE2.
Lane #1 is medium from astrocytes producing ApoE2.
Lane #2 is medium from astrocytes producing ApoE4.
The intense band is around 38 kDa.
The concentration used is 1ug/ml.