Antibody works very nicely for ICC. We use rat primary neuronal cultures, PFA fixation and antibody incubation over night at 4°C. 1:500 dilution of recommended reconstitution.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On April 13, 2014Simon Mølgaard, Aarhus University wrote:
5
This was tested on cultured hippocampal neurons at a concentration of 1:100. This antibody worked very well.
On June 3, 2019 Julia Bär wrote:
Antibody works very nicely for ICC. We use rat primary neuronal cultures, PFA fixation and antibody incubation over night at 4°C. 1:500 dilution of recommended reconstitution.
On April 13, 2014 Simon Mølgaard, Aarhus University wrote:
This was tested on cultured hippocampal neurons at a concentration of 1:100. This antibody worked very well.