Iba1 rabbit pAb Wako 019-19741

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Iba1
Mouse cortical glia stained with anti-Iba1 (red) and Hoechst (blue). Image provided by Simon Glerup, Aarhus University.

Iba 1 MethodsX
Ghatak et al. MethodsX 2014 show that Iba-1 signal using this antibody is increased following antigen retrieval using EDTA, pH 6.0.

See list of additional Iba1 antibodies at Linscott’s Directory

5 User Reviews

  • Anti-Iba1, rabbit polyclonal antibody from Wako Chemicals (Cat. No. – 019-19741)

    Used on frozen 50-micrometer thick human skin sections.
    Worked quite well in 1:500 dilution over night (immunofluorescence) with positive stainings both in epidermis and dermis of the skin.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
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    Flow cytometryNot Rated
    LuminexNot Rated
  • The Iba1 antibody is very robust in formalin-fixed paraffin-embedded tissue. It stains not only microglia but multiple macrophage-type cells in many tissues (intestinal lamina propria macrophages, Kupffer cells in liver, splenic red pulp macrophages, etc…). We use heat retrieval (pressure cooker) in pH6 buffer, incubate the primary for 1 hour at room temperature (around 1:5000 dilution), and use an HRP-linked secondary and DAB chromogen. There can be some punctate non-specific staining in nuclei, but you can get rid of most of it by diluting the antibody down.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
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    LuminexNot Rated
  • The image above shows selective Iba1 staining of primary mouse cortical glia. The signal seems very specific but one could wish for higher sensitivity.
    Cells were permeabilized in PBS+0.1% TritonX100 and blocked in PBS+10% FBS.
    Dilution for IF 1:500

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
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    LuminexNot Rated
  • Used for IF on cultured murine microglia, 1:500. Worked very well and seems specific for microglia. Also used on spinal cord sections, equally well, with clear increase in Iba1+ cells ipsilateral to sciatic injury.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
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    LuminexNot Rated
  • This Iba1 antibody is a great marker for microglia.
    It specifically stains these and not other glia in PFA fixed cryosections of mouse brain.
    It does however faintly stain nuclei of neurons in our hands.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
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ELISA
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Luminex