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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM

4 Pending Reviews

  • Glutamine Synthetase
    Polyclonal Guinea pig anti-glutamine synthetase antibody
    SySy cat. no 367 005

    The antibody was used 1:200 (stock concentration 1mg/ml) on mouse dorsal root ganglion tissue for IHC (obs: pretreatment needed – see further down).

    Prior to staining the tissue had been fixed in 4% PFA, cryoprotected in 30% sucrose, embedded in TissueTek and cut in 10um sections.

    Pretreatment: The slides with tissue sections were baked for 30 min at 60 degrees Celsius. Next, the tissue sections were treated with hydrogen peroxide for 10 min at room temperature. Without the hydrogen peroxide treatment I did not get any positive signal.

    The staining looked very specific to the satellite glial cells as expected.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • VAChT
    SySy #139 103
    rabbit polyclonal, affinity purified, K.O. verified

    works perfectly at mouse retina slides, dilution 1:500 (in PBS containing 0.1% Triton X and 10% of serum)

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • anti GAPDH (Santacruz #SC-36506)

    PBMC lysate
    WB: bis-tris+MOPS buffer, 1h 5% milk block, 1:10000 dilution

    Single intense band of appropriate MW size.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Anti-GSTA4 antibody produced in rabbit
    SAB2100981

    We used this in western blot and it didn’t work, we further tried this in immunostaining and unfortunately there it din’t work too. The tissue used was human.

    Western blot11111
    Immunostaining11111
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated