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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM

13 Pending Reviews

  • Rab 27 A – 168 013 (Synaptic Systems)
    This antibody works well for immunostaining Rab27A in primary hippocampal neurons from mice. 7 DIV neurons were fixed in 4% PFA and immunostained for Rab27B using 1:500 dilution in blocking buffer. Some background was observed.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Rab 27 B – 168 103
    This antibody works well for immunostaining Rab27B in primary hippocampal neurons from mice. 7 DIV neurons were fixed in 4% PFA and immunostained for Rab27B using 1:500 dilution in blocking buffer. Little background was observed.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • EEA1 – 237002 (Synaptic Systems)
    This antibody works excellent to detect Early Endosomes in primary hippocampal neurons. 7 DIV old neurons were fixed in 4% PFA and subsequently stained. A very clear signal was obtained with almost no background signal.

    Dilution 1:1000

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • SySy #224 102 GABA-A receptor a2
    This antibody did not work well for me. The antibody was tested on lysates from 11DIV primary cortical neurons from mice. A very faint band was observed after a very long exposure time using ECL Prime (Amersham), however the signal was too weak to quantify.

    Other antibodies were tested as well with great results (e.g. GABA(A) RECEPTOR SUBTYPE ALPHA 1, SYNAPTIC SYSTEMS # 224 203) on the same lysates.

    Western blot22222
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • SySy GluA2 – 182 103
    This antibody is really good for detection of GluA2 in primary cortical neurons from mice. Very sharp bands appeared after short exposure time using ECL Prime. Two bands occur as this antibody cross reacts with GluA3 as well.

    Dilution 1:1000

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • SySy 182 011 GluA1
    This antibody works great to detect GluA1 in primary cortical neurons from mice. Sharp bands were observed following BDNF treatment for 3 days (8-11DIV). The bands were developed using ECL prime with exposure time of 5-10 min.

    Dilution 1:1000

    Western blot44444
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • EAAT 3 cytoplasmic domain – 250 313
    This antibody works well in detecting EAAT3 (excitatory amino-acid transporter 3) in lysates from primary murine cortical neurons. A very short exposure time were needed to develop bands using this antibody, however a lot of unspecific bands also appeared along with a smear across the wells.

    Dilution 1:1000

    Western blot33333
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • GluN2B 244 103 SySy

    This antibody was tested on 11DIV primary cortical neurons from mice. Contrary 244 003 GluN 2 A/B, which worked well on the same samples, this antibody did not work in detecting GluN2B.

    Dilution 1:1000

    Western blot11111
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • GluN 2 A/B – 244 003 SySy
    This antibody works well to detect GluN2A (NR2A) in 11DIV primary cortical neurons from mice. This antibody also cross-reacts to GluN2B due to sequence homology.

    A sharp band was observed at approx. 170kDa, however only after longer exposure time (10min +) using ECL prime (Amersham).

    Dilution 1:1000

    Western blot44444
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • ATF 4 – 318 003 (synaptic systems)
    This antibody works very well in detecting ATF4 (CREB2) of lysates from murine primary cortical neurons.
    A double band is detected using this (approx. 50kDa and 45kDa) appeared after 1 min exposure. The upper band at 50kDa is ATF4, whereas the 45kDa lower band is unspecific.

    Dilution 1:1000.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • MAB5374 – Anti-Huntingtin Protein Antibody, clone mEM48
    I used this antibody to stain murine 50um thick brain slices ( 4% PFA fixed brains). Prior to immunohistochemistry, the tissue were target retrieved using DAKO antigen retrieval s1699 according to protocol from the manufacturer.

    This antibody works excellent for detection of aggregated mutant Huntingtin in mice suffering from Huntington’s. Nuclear aggregations were clearly detected using this antibody with very low background signal.

    Dilution 1:50 (overnight incubation)

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Glutamine Synthetase
    Polyclonal Guinea pig anti-glutamine synthetase antibody
    SySy cat. no 367 005

    The antibody was used 1:200 (stock concentration 1mg/ml) on mouse dorsal root ganglion tissue for IHC (obs: pretreatment needed – see further down).

    Prior to staining the tissue had been fixed in 4% PFA, cryoprotected in 30% sucrose, embedded in TissueTek and cut in 10um sections.

    Pretreatment: The slides with tissue sections were baked for 30 min at 60 degrees Celsius. Next, the tissue sections were treated with hydrogen peroxide for 10 min at room temperature. Without the hydrogen peroxide treatment I did not get any positive signal.

    The staining looked very specific to the satellite glial cells as expected.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Anti-GSTA4 antibody produced in rabbit
    SAB2100981

    We used this in western blot and it didn’t work, we further tried this in immunostaining and unfortunately there it din’t work too. The tissue used was human.

    Western blot11111
    Immunostaining11111
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated