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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM

4 Pending Reviews

  • Good evening guys, I did a comparison between the mAb anti-M13:HRP from GE with a brand new version from Santa Cruz Biotech (SC-53004-HRP). Unfortunately the one that everybody has been using from GE it is discontinued so the Santa Cruz seems to be a good reliable alternative.

    Basically it works as good (or even better) as the one from GE at the same dilution. I also did the titration curves of the same amount of phages,and decreasing amounts of the secondary antibody to evaluate it performance and it was really good. It can be diluted up to 1/20.000 to get a signal of 1.

    The product is available at 200 ug/mL and It worked very nicely at 1/5K dilution (100 uL/well) sc-53004 HRP for 500uL. It seems to be a really good alternative.

    In researchgate I uploaded the graph:
    https://www.researchgate.net/post/anti-M13_pVIIIHRP

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISA55555
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • ULEX (Europaeus agglutinin I) from Vector Laboratories (Burlingame, CA, USA).

    Concentration: 1:100

    When it works, it stains beautifully epidermal keratinocytes, epithelia and endothelia of blood vessels. However, the staining does not always work as all I can see is unspecific background staining.

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Rabbit dopamine beta hydroxylase (DbH) antibody, used in immunostaining (fluorescence).
    Concentration: 1:150
    Manufacturer: Chemicon/Millipore
    Product ID: B-1065

    Works wonderfully to visualize arrector pili innervation/pilomotor nerve fibers in human skin tissue (cut 50 micrometer thick cryosections). Antibody left on over-night on shaker table and secondary antibody for 2 hours.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Collagen IV – rabbit anti-human from Biodesign
    Used on eyes from mice to stain lens capsule.
    Concentration 1:100

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated