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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM

8 Pending Reviews

  • Rab7 (D95F2) XP® Rabbit mAb #9367
    This antibody works great to detect Rab7 in primary cortical neuronal cultures from mice. A very clear signal was obtained with little to no background signal.
    The neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:100 dilution in blocking buffer o.n.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Rab11 (D4F5) XP® Rabbit mAb #5589
    This antibody works great to detect Rab11 in primary cortical neuronal cultures from mice. A very clear signal was obtained with little to no background signal.
    The neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:100 dilution in blocking buffer o.n.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8) Rabbit mAb #4168 Cell Signaling Technology

    This antibody worked well to detect phosphorylation of FL TrkB in murine primary hippocampal and cortical neuronal lysates. Clear bands were visible when stimulating the neurons with 1nM BDNF compared to non-stimulated. Bands were detected using Amersham ECL Prime and imaging for 20 min.

    WB Dilution 1:1000

    Western blot33333
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • CD4 (human), mouse mAb (clone SK3), BV510 conjugated, BD Biosciences, 562970

    Used to stain lymphocytes in an activation panel along with (CD8 and CD137).
    2uL used to stain cells in a FACS tube at 4 degrees.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometry44444
    LuminexNot Rated
    EMNot Rated
  • CD8 (human), mouse mAb (clone SK1), PerCP conjugated, BD Biosciences, 345774

    Used to stain lymphocytes in an activation panel along with (CD4 and CD137).
    3uL used to stain cells in a FACS tube at 4 degrees.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometry22222
    LuminexNot Rated
    EMNot Rated
  • ULEX (Europaeus agglutinin I) from Vector Laboratories (Burlingame, CA, USA).

    Concentration: 1:100

    When it works, it stains beautifully epidermal keratinocytes, epithelia and endothelia of blood vessels. However, the staining does not always work as all I can see is unspecific background staining.

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Rabbit dopamine beta hydroxylase (DbH) antibody, used in immunostaining (fluorescence).
    Concentration: 1:150
    Manufacturer: Chemicon/Millipore
    Product ID: B-1065

    Works wonderfully to visualize arrector pili innervation/pilomotor nerve fibers in human skin tissue (cut 50 micrometer thick cryosections). Antibody left on over-night on shaker table and secondary antibody for 2 hours.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Collagen IV – rabbit anti-human from Biodesign
    Used on eyes from mice to stain lens capsule.
    Concentration 1:100

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated