GAPDH mouse Sigma G8795

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6 User Reviews

  • Excellent control for HepG2 cell lysates. Reused +25 times and diluted 1:5000

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Great loading control for HepG2 cell lysates. Single band detected at 38 kDa marker band (See blue Plus2 marker). We dilute at least 1:2000 and re-use several times.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This was tested on lysates from hippocampal tissue. Works excellent for detection of GAPDH! Also works for reprobing stripped membranes. Was used at a dilution of 1:5000

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Superior antibody to probe as this house keeping gene is less susceptible to modulation by extraneous variable (except changes in mitochondrial function) whereas other common loading controls such as actin and tubulin can be altered by many different variables. I have used this antibody on cell lysates from primary cortical neuronal cultures and motor neuron cultures from nice, also human iPSC derived cells and human ES cells. Works great. 1:5000 for WB, more than enough for 2H at room temperature.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody is not good for Western blotting of lysates obtained from cross-linked (DSP) HEK cells

  • Excellent for Western blotting 1:5000

    Can also be used for reprobing stripped membranes.

    Superb as loading control. May be superior to beta-actin.

    Beta-actin levels are affected dramatically by e.g. insulin-induced signaling cascades.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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