Related topics: Alphabetical list, Metabolism
See list of additional GAPDH antibodies at Linscott’s Directory
On December 13, 2018
Kasper Budolph Pedersen wrote:
Excellent control for HepG2 cell lysates. Reused +25 times and diluted 1:5000
On June 22, 2017
Camilla Gustafsen, Aarhus Universitet wrote:
Great loading control for HepG2 cell lysates. Single band detected at 38 kDa marker band (See blue Plus2 marker). We dilute at least 1:2000 and re-use several times.
On August 27, 2015
Simon Mølgaard wrote:
This was tested on lysates from hippocampal tissue. Works excellent for detection of GAPDH! Also works for reprobing stripped membranes. Was used at a dilution of 1:5000
On November 24, 2014
Melissa Wren wrote:
Superior antibody to probe as this house keeping gene is less susceptible to modulation by extraneous variable (except changes in mitochondrial function) whereas other common loading controls such as actin and tubulin can be altered by many different variables. I have used this antibody on cell lysates from primary cortical neuronal cultures and motor neuron cultures from nice, also human iPSC derived cells and human ES cells. Works great. 1:5000 for WB, more than enough for 2H at room temperature.
On August 6, 2013
Camilla Gustafsen wrote:
This antibody is not good for Western blotting of lysates obtained from cross-linked (DSP) HEK cells
On February 13, 2012
Simon Glerup wrote:
Excellent for Western blotting 1:5000
Can also be used for reprobing stripped membranes.
Superb as loading control. May be superior to beta-actin.
Beta-actin levels are affected dramatically by e.g. insulin-induced signaling cascades.
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