Latest Antibody Reviews

Review date: 2019-03-26 10:52

GAPDH, mouse mAb clone G-9, Santa Cruz, sc-365062

Western blot

PBMC lysate WB: bis-tris+MOPS buffer, 1h 5% milk block, 1:10000 dilution Single intense band of appropriate MW size.

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Review date: 2019-03-14 15:53

Anti-mouse IgG F(ab’)2 fragment specific, goat pAb, Alexa Fluor-647 conjugated, Jackson ImmunoResearch, 115-605-006

Flow cytometry

AF647-F(ab’)2 antibody used in a titration experiment to stain CART19 cells expressing a chimeric antigen receptor or to stain negative...

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Review date: 2019-03-14 15:20

Beta-Actin mAb clone AC-15 Sigma A5441

Western blot
Immunostaining
IP

This antibody works great to visualize the cytoskeleton of HEK293 cells. Dilution 1:1000.

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Publishing

pAbmAbs collaborates with the Elsevier journal MethodsX and the Faculty of 1000 journal F1000Research.

Click here for example of F1000Research paper by Molgaard et al. on data extracted from pAbmAbs

Click here for example of MethodsX paper on pAbmAbs data

Competitions

12th pAbmAbs competition
All reviews submitted After July 6th will enter the draw for 500 Euro.

Previous winners
July 6, 2016. Signe Petersen, Aarhus University. Click to watch the video.
February 29, 2016. Rachel L. Flynn, Boston University school of Medicine. Click to watch the video.
November 30, 2015. Kasper M. Jensen, Aarhus University. Watch the video of the process here
July 30, 2015. Piotr Siupka, Aarhus University
May 18, 2015. Julia Abele, University of Magdeburg
March 31, 2015. Noemie Tentillier, Aarhus University.
February 15, 2015. Canan Doganli, UCSF
December 24, 2014. Julie Torvund-Jensen, Aarhus University
May 31, 2014. Peter Lund Ovesen, Aarhus University
March 21, 2014. Stine Klinger, NIH, Washington
December 20, 2013. Mads Kjolby, Aarhus University

About pAbmAbs

This site is made to share antibody experiences – good and bad – with the scientific community.

In our lab, we spend a fortune each year on commercial research antibodies that do not work as promised in their data sheets. However at present, it is very difficult to extract such information from the published litterature since negative results are seldom published.

The site is independent and free from any commercial interests whatsoever.

Read about pAbmAbs in Labtimes
Watch pAbmAbs presentation at 1st International Antibody Validation Forum at University of London

Simon Glerup
Associate Professor
glerup@pabmabs.com
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Camilla Gustafsen
Assistant Professor
gustafsen@pabmabs.com
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Simon Mølgaard
Postdoc
molgaard@pabmabs.com
LinkedIn