Related topics: Alphabetical list, Apoptosis, Cancer biology, Cell cycle, Signal transduction
See list of additional phospho-MAPK antibodies at Linscott’s Directory
On September 12, 2017
Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University wrote:
This antibody was used for immunoblotting of RIPA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
On January 23, 2017
Christina Demuth wrote:
This antibody is used for evaluating activity of the EGF-receptor pathway after stimulation or inhibition of EGFR in lung cancer cell lines.
On April 17, 2015
Maj Ulrichsen wrote:
I have used this antibody for detection of phospho-MAPK in lysates (50 ug total protein) of unstimulated and NT3-stimulated starved rat Schwann cells. It works great as I only see bands in the stimulated cells. Dilution 1:1000 of stock.
On April 15, 2015
Lars Meinertz Byg wrote:
This antibody worked very well for detecting differences in ERK 1/2 phosphorylation following stiumulation with BDNF of Sorcs2/trkB transfected SY5Y-cell lines. There was however also faint bands in the nonstimulated cells.
On March 13, 2015
Simon Mølgaard wrote:
I have used this antibody for IHC on Insulin-stimulated HEK293 cells as well as BDNF-stimulated hippocampal neurons. Works great! Showed high signal intensity and almost no background in the negative control. Dilution 1:250.
On January 16, 2015
Jakob Vejby Larsen wrote:
I have used this antibody in stimulated murine primary macrophages and 293 cells. It works great! Dilution 1:1000
On January 8, 2015
Mads Kjolby, Aarhus University wrote:
I have used this antibody for detection of phospho-MAPK in stimulated murine primary macrophages. I have used 1:1000 of stock in WB.