Related topics: Alphabetical list, Cellular marker proteins, Neuroscience, Neurotransmission
On October 29, 2019
Angelica Foggetti, Christian-Albrechts-Universität zu Kiel wrote:
Works extremely well for immunofluorescence in mouse brain sections. Brains were perfused and post-fixed overnight with 4% paraformaldehyde. Sections were 50-80 μm thick and permeabilized for 30-60 minutes.
Dilution used: 1:5000
For more information see Foggetti et al., Spiny and Non-spiny Parvalbumin-Positive Hippocampal Interneurons Show Different Plastic Properties, Cell Reports, vol 27, issue 13, p3725-3732, June 25, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30737-5).
On October 17, 2019
David I. Hughes, University of Glasgow wrote:
This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).