Shb/Shf (B-1), mouse mAb, Santa Cruz, sc-74483

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One User Review

  • This antibody was evaluated in a study of commercially available antibodies directed against SH2 domain-containing adapter protein B (Shb). Nine antibodies were included in the study and their performance was tested in Western blotting and Immunoprecipitation.

    Tests were performed using cell lysate obtained from the cell line HEK 293T, which endogenously expresses Shb, and HEK 293T cells transiently transfected with CMV promoter driven overexpression of Shb. As a negative control, Shb was knocked-down in HEK 293T-derived cell line CE12 using Shb-specific siRNA.

    This antibody proved to be the most specific one of the nine antibodies included in the test. It was clearly able to detect the endogenous Shb protein. The strongest signal corresponded to Shb and only one or two non-specific weaker bands were detected.

    The antibody was unable to immuneprecipitate the target.

    Immunogen: C-terminal amino acids (380 509) of human Shb
    Lot number: K0207
    Concentration: 2 µg/ml

    Results are summarized in inserted table.

    For further information see: Evaluation and validation of commercial antibodies for the detection of Shb. Vanli, Cuesta-Marban and Widmann, PLOS ONE December 1, 2017.

    Western blot44444
    ImmunostainingNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Flow cytometry