Related topics: Alphabetical list, Cell adhesion, Immunology, Neuroscience
See list of additional CD56 antibodies at Linscott’s Directory
On October 29, 2017
Kasper Mølgaard Jensen, Center for Cancer Immune Therapy (CCIT), Herlev Hospital, Denmark wrote:
Anti-human CD56, PE-conjugated, clone MY31, BD Bioscience #345810
By manufactures instruction this clone is not recommended for intracellular cytokine staining (ICS).
PE anti-CD56 was used in an antibody cocktail for ICS of PBMCs Expressing a chimeric antigen receptor.
Protocol in short: 3uL/sample added to antibody cocktail at a final volume of 20uL. 20uL antibody cocktail added to cells to a final volume of approximately 30uL. Cells incubated in dark for 20 minutes before washing, fixation, and intracellular staining.
Indeed, anti-CD56 clone MY31 did not work in ICS for identifying CD3-/CD56+ NK cells.