Related topics: Histon methlyation
Veerle_results – test SUV420H1 AB
See list of additional SUV420H1 antibodies at Linscott’s Directory
On February 24, 2017
Veerle Paternoster wrote:
Rabbit anti-SUV420H1 (Origene TA335571), 1:2500 in 5 ml.
Different sample preparations (all mouse brain) and different blocking agents.
A very faint band of approximate MW appears when overexposing. Lower MW isoforms are not covered by the epitope, so these must be aspecific bands.
Please find link to image above.
On January 5, 2017
Veerle Paternoster, Aarhus University wrote:
Loaded: extract from HEK293T cells, mouse brain and pig brain. Several bands visible, though not at expected MW of canoncical form (99kDa). Bands may represent shorter isoforms.
WB blocked with milk, 1AB overnight incubation at 4*C.
Please see image above.