Related topics: Alphabetical list, Antibody detection, Epitope tags
See list of additional c-myc antibodies at Linscott’s Directory
On November 6, 2015 Camilla Gustafsen, Aarhus University wrote:
Myc epitope tag clone 9E10 mAb, mouse IgG2A Absolute Antibody Ab00100-2.0
I have used this antibody (1:1000) for WB detection of 1µg Myc-tagged protein. A very strong band was seen. As negative control was used un-tagged protein. I am sure that the antibody can be diluted more, and less protein can be loaded.
2. Ab: HRP-anti Mouse IgG, Cell Signaling 7076S (1:2000), 1 hour at RT
Similar band-intensity was seen with Ab00100-1.1
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On November 6, 2015 Camilla Gustafsen, Aarhus University wrote:
Myc epitope tag
clone 9E10
mAb, mouse IgG2A
Absolute Antibody
Ab00100-2.0
I have used this antibody (1:1000) for WB detection of 1µg Myc-tagged protein. A very strong band was seen. As negative control was used un-tagged protein. I am sure that the antibody can be diluted more, and less protein can be loaded.
2. Ab: HRP-anti Mouse IgG, Cell Signaling 7076S (1:2000), 1 hour at RT
Similar band-intensity was seen with Ab00100-1.1