Related topics: Alphabetical list, Alzheimer's disease, Neurodegeneration, Protein trafficking
See list of additional SorLA antibodies at Linscott’s Directory
On December 15, 2014
Laura Johnsen wrote:
I have used the SorLA antibody 20:C11 on cryosectioned, PFA fixated mouse dorsal root ganglia, on which it works fine.
I have used it in combination with Fab buffer (Donkey anti-mouse)
On April 11, 2014
Thaneas Prabakaran, Aarhus University wrote:
It works very well for immunostaining of PFA fixed cells at a dilution of 1:100
On March 7, 2014
Helene Andersen wrote:
I have used this antibody for immunostainings on adult mouse barin tissue. It worked very well for detection of SorLA.
On January 29, 2014
Simon Mølgaard wrote:
I have used this antibody for immunostainings on adult mouse barin tissue as well as cultured hipoocampal neurons. In both cases this antibody worked very well for detection of SorLA.
On December 10, 2013
Mathias Kaas Ollendorff wrote:
We used this SorLA mAb for ELISA on human serum. Development takes about 20-30 minutes, gives a nice std curve and good consistent measurements for serum diluted 5, 10 and 20 times in 1 % BSA.
For best results we used Nunc MaxiSorp 96 well plates.
Concentration was 1 ug/ml
On December 2, 2013
Mads Kjolby wrote:
I have used the 20C11 antibody for staining of brain sections from cortex (nice neuron staining in vesicle strucures / TGN) and also in dopaminergic neurons in substantia nigra in mesencephalon (see Glerup et al., Cell reports, 2013) (double staining with anti-TH and SorLA).
OBS: In mice it is necessary to block with fab fragments if the tissue has to much background.
On August 20, 2013
Stine Klinger wrote:
Not reactive against rat SorLA
On December 21, 2011
Simon Glerup, Aarhus University wrote:
This is a nice SorLA mAb produced in Claus M. Petersen lab, Aarhus. Cited in Klinger et al. Journal of Cell Science 2011
I have used it for Western blotting on brain from wt and SorLA knockouts. It detects a specific band around 250 kDa.
It is great for staining glia and neurons both in ICC and IHC. Knockouts are blank.
For WB 1 ug/ml
For ICC 10 ug/ml
The image above shows a primary mouse cortical astrocyte stained using SorLA mAb 20C11 (green) and GFAP (red, DAKO)