Rubicon, Cell Signaling Technology, 8465

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Yamamuro et al. PLOS genetics, 2021.
Yamamuro et al. validate this antibody for detection of mouse Rubicon by Western blotting of mice testes cell lysate. No band was observed in testes Rubicon-KO mice (Supplementary figure 1A).
Based on the available data, we have assigned a five-star rating because of strong and clean signal from all three lanes with RubiconKO, but we highly encourage other scientists to share their observations.