TAK1, Cell Signaling Technology, 5206

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Wang et al. Cancers 2021
Wang et al. validate this antibody for detection of endogenous human TAK1 by Western blotting of ES-2 (clear cell subtype ovarian cancer cell line) and MES-OV cells (serous subtype ovarian cancer cell lines). The figure shows a clear reduction or loss of the expected TAK1 band in the KO lanes. However, there are other bands and some background signal present on the blot(s) (figure 4f)
Based on the available data, we have assigned a four-star rating, but we highly encourage other scientists to share their observations.