HIF-1a, Abcam, ab179483

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Wang et al. Cell Death and Disease 2021.
Wang et al. validate this antibody for detection of endogenous human HIF-1a by immunostaining of GMB (glioblastoma) primary cells. Furthermore Wang et al. validate this antibody by western blotting of GMB-1 and GMB-3 cells. No staining was observed in HIF1a-ko cells (figure 3a) and no band was observed in either GMB-1 or GMB-3 HIF1a-KO (Figure 3c).
Based on the available data, we have assigned a five-star rating to both Immunostaining and Western blotting, but we highly encourage other scientists to share their observations.