Caveolin-1, Cell Signaling Technology, 3267

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Caveolin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 17 commercial research antibodies against human Caveolin-1 encoded by CAV1.

The collection of antibodies was tested using U-87 MG and CAV1 KO cells in the application WB (WT and KO cell lysates/20 µg protein), according to YCharOS standard protocols (see reference).

In WB (1:1000) 3267 detected a band of appr. 20 kDa in WT lysate, with no corresponding band observed in KO lysate.

We highly encourage other scientists to share their observations.

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Jozic et al. Communication Biology 2021
Jovic et al. validate this antibody for detection of endogenous human Caveolin-1 (Cav-1) by Western blotting of human keratinocytes. No band was observed in Cav-1 KO keratinocytes (Fig. 2d). Full gel image available in the Supplementary information (Supplementary Figure 7).
Based on the available data, we have assigned a five star rating but we highly encourage other scientists to share their observations.