WCH4, Alomone Labs, AQP-004
What do reviewers generally think about AQP-004 from Alomone Labs?
Researchers agree that WCH4, Alomone Labs, AQP-004 performs well for IHC on paraffin-embedded whole mouse eye sections, giving clear AQP4 staining at 1:1600, including in ciliary body non-pigmented epithelial cells. One reviewer points out that no background was observed when blocking with 1% milk and using an Alexa 488 donkey anti-rabbit secondary antibody. However, another reviewer reports no signal by Western blotting of whole kidney homogenates at 1:1000, suggesting limited applicability outside IHC.
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User Reviews (2)
Review of: Aquaporin 4, Alomone Labs, AQP-004 by Tina Storm, Aarhus University
Rabbit anti AQP4 (249-323)(raised agaisnt peptide of rat origin)is excellent for detection of AQP4 in paraffin embedded whole mouse eye sections(perfusion fixed with 2 % PFA). We used a dilution of 1:1600 and visualized with Alexa donkey a rabbit fluorophore coupled (488) antibody. No background observed with 1 % milk blocking.
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0 not usefulReview of: Aquaporin 4, Alomone Labs, AQP-004 by Kathrin Weyer
Anti-Aquaporin 4 (249-323) from Alomone labs.
This is a polyclonal rabbit anti-rat antibody (raised against aa residues 249-323 of rat AQP4).
In IHC of murine whole eye paraffin sections it gives nice staining in non-pigmented epithelial cells of the ciliary body (1:1600 concentration). However, no signal was seen by Western blotting of whole kidney homogenates (1:1000).
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