Lamin C, Developmental Studies Hybridome Bank, AB_528339
What do reviewers generally think about AB_528339 from Developmental Studies Hybridoma Bank?
Researchers report consistent success with Lamin C, Developmental Studies Hybridome Bank, AB_528339 for Drosophila immunostaining, including wing disc immunostaining, IHC on 4% PFA-fixed embryos, and ICC on larval imaginal discs. One reviewer points out that a 1:100 dilution after 30 minutes fixation in 4% formaldehyde at room temperature gave great results. Another notes a 1:50 dilution works well for larval imaginal disc ICC. No complaints are mentioned.
Information about this antibody
Vendor notes
User Reviews (3)
Review of: Lamin C, Developmental Studies Hybridome Bank, AB_528339 by Maria Paula Zappia, University of Illinois at Chicago
Used for immunostaining of drosophila wing discs. 30 minutes fixation in 4% formaldehyde at room temp. Great result.
Dilution used: 1:100
For more information see Zappia et al. EMBO Reports 2020, “A cell atlas of adult muscle precursors uncovers early events in fibre‐type divergence in Drosophila”.
https://www.embopress.org/doi/full/10.15252/embr.201949555
0 useful
0 not usefulReview of: Lamin C, Developmental Studies Hybridome Bank, AB_528339 by Tomoki Ishibashi
We used this antibody for IHC staining of 4% PFA-fixed Drosophila embryo, and it works very well.
Comments
0 useful 0 not usefulReview of: Lamin C, Developmental Studies Hybridome Bank, AB_528339 by Bing Zhang initiative
Works great for ICC on fly larval imaginal discs. Dilution: 1:50
Comments
0 useful 0 not useful
Comments