Cleaved Drosophila Dcp-1 (Asp216), Cell Signaling Technology, 9578
What do reviewers generally think about 9578 from Cell Signaling Technology?
Researchers generally report that Cleaved Drosophila Dcp-1 (Asp216) works well for Drosophila immunostaining, including IHC on L3 wing discs and ICC on eye and larval imaginal discs, often at 1:500 or 1:100, with one successful wing-disc protocol using 30 min 4% formaldehyde at room temperature. One reviewer points out that unspecific signals can appear in UAS-p35 flies, possibly due to sequence similarity near Asp216, though the antibody is otherwise used frequently and performs fine. Another notes initial background required optimization before results were good.
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User Reviews (5)
Review of: Cleaved Drosophila Dcp-1 (Asp216), Cell Signaling Technology, 9578 by Maria Paula Zappia, University of Illinois at Chicago
Used for immunostaining of drosophila wing discs. 30 minutes fixation in 4% formaldehyde at room temp. Great result.
Dilution used: 1:500
For more information see Zappia et al. EMBO Reports 2020, “A cell atlas of adult muscle precursors uncovers early events in fibre‐type divergence in Drosophila”.
https://www.embopress.org/doi/full/10.15252/embr.201949555
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0 not usefulReview of: Cleaved Drosophila Dcp-1 (Asp216), Cell Signaling Technology, 9578 by Ugurcan Sakizli
I see some unspecific signals when I use it with UAS-p35 flies, which may be caused by some unlucky sequence similarity with “amino-terminal residues adjacent to Asp216”. Other than that we use it frequently, seems to be working fine with IHC on L3 wing discs.
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0 useful 0 not usefulReview of: Cleaved Drosophila Dcp-1 (Asp216), Cell Signaling Technology, 9578 by Bing Zhang initiative
Works well for ICC on fly eye discs. Dilution: 1:100
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0 useful 0 not usefulReview of: Cleaved Drosophila Dcp-1 (Asp216), Cell Signaling Technology, 9578 by Bing Zhang initiative
Works great for ICC on fly larval imaginal discs. Dilution used: 1:500
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0 useful 0 not usefulReview of: Cleaved Drosophila Dcp-1 (Asp216), Cell Signaling Technology, 9578 by Jimena Berni, University of Cambridge
It took us a bit of time to make it work because there was a bit of background . Once we found the conditions it was quite good.
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