α-Tubulin, Sigma-Aldrich, T5168
What do reviewers generally think about T5168 from Sigma-Aldrich?
Researchers report that the α-Tubulin antibody performs well for western blotting of cell lysates and mouse hippocampal tissue, with fine results in immunofluorescence. One reviewer points out that they use it at a 1:10,000 dilution and often rely on it as a control for their blotting protocol. No complaints are mentioned.
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User Reviews (2)
Review of: α-Tubulin, Sigma-Aldrich, T5168 by Roux Philippe
Used for WB on cell lysates.
Fine result in IF.
For more information see Nourreddine et al. Cell Reports 2020, “NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay”.
https://www.sciencedirect.com/science/article/pii/S2211124720306136#
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0 not usefulReview of: α-Tubulin, Sigma-Aldrich, T5168 by Tiago Gil Oliveira, University of Minho
I have used this antibody for western blotting of total lysates from mice hippocampal tissue in a dilution of 1:10000. This antibody works so well for me, that I often use it as a control of my blotting protocol.
For more information see Santa-Marinha et al. Cell Reports 2020, “Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning”
https://www.sciencedirect.com/science/article/pii/S2211124720302837#sec4
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