p53, Santa Cruz Biotechnology, sc-126

We generated a top 1000 list of the most studied and most published proteins and analyzed the literature validation data for the commercially available research antibody tools. Mircetic et al., Nature Communications 2017 validates sc-126 for IF in Figure 2b in WT-2G and KO-2G cells, showing specific staining of WT cells, not present in KO cells. The authors also demonstrate the detection of a specific band in WT cells, not present in KO cells (Figure 2c, uncropped blots in Supplementary Figure 9). We have rated sc-126 based on the available information and according to the pAbmAbs criteria.

Good signal in western blot. Dilution 1:4000 in 1% milk in PBS-T

For more information see Rizzotto et al. Cell Reports 2020, “Nutlin-Induced Apoptosis Is Specified by a Translation Program Regulated by PCBP2 and DHX30”. https://doi.org/10.1016/j.celrep.2020.03.011