GAPDH, Sigma-Aldrich, G9545
What do reviewers generally think about G9545 from Sigma-Aldrich?
Researchers report that the GAPDH antibody performs well in western blotting of mouse samples, including hippocampal tissue lysates and primary microglia lysates, with dilutions of 1:3000 and 1:1000. It is used as a loading control for protein normalization. One reviewer points out that it works so well in their lab for western blotting of hippocampal tissue lysates from C57BL/6J mice. No complaints are mentioned.
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User Reviews (2)
Review of: GAPDH, Sigma-Aldrich, G9545 by Shirin Hosseini, Technische Universität Braunschweig
I have used this antibody for western blotting of tissue lysates from hippocampus of C57BL/6J mice in a dilution of 1:3000. This antibody works so well in our lab. We often use it in our western blotting as a loading control for protein normalization.
For more information see Hosseini et al. Cell Reports 2020, “Type I Interferon Receptor Signaling in Astrocytes Regulates Hippocampal Synaptic Plasticity and Cognitive Function of the Healthy CNS”.
https://www.sciencedirect.com/science/article/pii/S2211124720306197
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0 not usefulReview of: GAPDH, Sigma-Aldrich, G9545 by Lea Friker, University of Bonn
This antibody worked great for WB of primary mouse microglia lysates.
Dilution used: 1:1000
For more information see Friker et al. Cell Reports 2020, “β-Amyloid Clustering around ASC Fibrils Boosts Its Toxicity in Microglia”.
https://www.sciencedirect.com/science/article/pii/S2211124720301868
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