VGLUT1, Millipore, AB5905
What do reviewers generally think about AB5905 from Sigma-Aldrich?
Researchers report that VGLUT1, Millipore, AB5905 works extremely well for immunofluorescence in perfusion-fixed mouse brain sections and for immunostaining of perfusion-fixed mouse tissue by fluorescence microscopy, using a 1:5000 dilution. One reviewer points out that 50-80 μm sections permeabilized for 30-60 minutes after overnight 4% paraformaldehyde fixation were used successfully. No complaints are mentioned.
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User Reviews (2)
Review of: VGLUT1, Millipore, AB5905 by Angelica Foggetti, Christian-Albrechts-Universität zu Kiel
Works extremely well for immunofluorescence in mouse brain sections. Brains were perfused and post-fixed overnight with 4% paraformaldehyde. Sections were 50-80 μm thick and permeabilized for 30-60 minutes.
Dilution used: 1:5000
For more information see Foggetti et al., Spiny and Non-spiny Parvalbumin-Positive Hippocampal Interneurons Show Different Plastic Properties, Cell Reports, vol 27, issue 13, p3725-3732, June 25, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30737-5).
0 useful
0 not usefulReview of: VGLUT1, Millipore, AB5905 by David I. Hughes, University of Glasgow
This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:5000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
Hughes Laboratory:
https://specciebear.wixsite.com/spinal-cord-hughes
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