CNOT7, Cell Signaling Technology, 86665

This antibody works excellent for detecting CNOT7 in lysates from mouse hippocampal tissue. Very clear bands developed after short exposure. The tissue was lysed in lysis buffer described below using TissueLyser II and followed by sonication.

Dilution 1:1000

Lysis buffer:

• 20 mM MOPS
• 2 mM EGTA (to bind calcium);
• 5 mM EDTA (to bind magnesium and manganese);
• 50 mM sodium fluoride (to inhibit protein-serine phosphatases);
• 60 mM β-glycerophosphate, pH 7.2 (to inhibit protein-serine phosphatases);
• 25 mM sodium pyrophosphate (to inhibit protein-serine phosphatases);
• 2.5 mM sodium orthovanadate (to inhibit protein-tyrosine phosphatases);
• 50nM phenylarsine oxide (to inhibit protein-tyrosine phosphatases);
• 1% Triton X-100
• 0.05% sodium dodecylsulphate (SDS)
cOmplete™ Protease Inhibitor and DTT added before use.