Beta 3 tubulin, Synaptic Systems, 302 304
What do reviewers generally think about 302 304 from Synaptic Systems?
Overall, the reviews are mixed. One reviewer points out that the antibody never worked for IF on samples confirmed MAP2 positive. Another reviewer describes it as very useful for detecting neurons and their processes, particularly with indirect Cy2 fluorescence in paraformaldehyde-frozen rodent brain sections, where it revealed fine morphological detail and was used to show stroke-related changes. Thus, it is praised as an outstanding neuronal IHC marker in one application, but criticized as nonfunctional in another IF setting.
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User Reviews (2)
Review of: Beta 3 tubulin, Synaptic Systems, 302 304 by Jamie Rose, University of Edinburgh
Used for IF. Never works. Does not work on samples confirmed MAP2 positive by SYSY188004.
For more information see Pickett et al. Cell Reports 2019, “Amyloid Beta and Tau Cooperate to Cause Reversible Behavioral and Transcriptional Deficits in a Model of Alzheimer’s Disease”.
https://www.sciencedirect.com/science/article/pii/S2211124719315268?dgcid=rss_sd_all#!
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0 not usefulReview of: Beta 3 tubulin, Synaptic Systems, 302 304 by Wolfgang Härtig, University of Leipzig
For the detection of neurons and especially their processes guinea pig-anti-βIII-tubulin (Synaptic Systems, Göttingen, Germany; 302304) might be considered as very useful antiserum. Applying indirect fluorescence staining with green fluorescent carbocyanine (Cy)2-tagged donkey-anti-guinea pig IgG this immunoreagent reveals fine morphological details in paraformaldehyde frozen sections of rodent brains. Recently, it was also used to demonstrate alterations resulting after experimentally induced stroke in mice (Aleithe et al., Mol Neurobiol, 2019, Fig. 3).
This antibody is an outstanding immunohistochemical marker
Aleithe et al., Mol Neurobiol, 2019: https://link.springer.com/article/10.1007/s12035-019-1604-4
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