LC3B, Novus Biologicals, NB100-2220
What do reviewers generally think about NB100-2220 from Novus Biologicals?
Researchers report that LC3B, Novus Biologicals, NB100-2220 performs well for detecting LC3-I and LC3-II by Western blot and immunocytochemistry, giving clear signals in mouse primary cortical and hippocampal neurons. Suggested dilutions are 1:1000 for Western blot and 1:1000 or 1:200 for ICC. One reviewer points out that 4% PFA fixation and 0.1% Triton permeabilization worked for primary hippocampal neurons. No complaints are mentioned.
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User Reviews (2)
Review of: LC3B, Novus Biologicals, NB100-2220 by Anders Dalby
This antibody works great for detection of LC3-I and LC3-II in primary neurons from mice (cortical). A clear signal was obtained after short exposure.
Dilution 1:1000
0 useful
0 not usefulReview of: LC3B, Novus Biologicals, NB100-2220 by Sérgio Almeida
This antibody works very well for WB detection of LC3-I and LC3-II. Dilution of 1:1000.
I was also able to do ICC of primary hippocampal neurons with this autophagosome marker, dilution 1:200. (4% PFA for fixation and 0.1% triton for permeabilization.)
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