Iba1, Synaptic Systems, 234 004
What do reviewers generally think about 234 004 from Synaptic Systems?
Researchers report Iba1, Synaptic Systems, 234 004 performs well in 4% PFA-fixed mouse brain slices and murine primary microglia ICC, and in human and mouse paraffin-embedded sections with citrate pH 6.0 antigen retrieval at 1:500, showing strong specificity and little or no background. One reviewer points out that slight background at 1:200 may be reduced by using 1:500.
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Review of: Iba1, Synaptic Systems, 234 004 by Isabel Groß
Worked well in 4% PFA perfusion-fixed mouse brain slices (vibratome, 30 µm). I had a little background staining, but I used it 1:200, might be reducable with 1:500 dilution.
Nice results also in ICC of 4% PFA-fixed murine primary microglia cultures with 1:500 dilution!
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0 not usefulReview of: Iba1, Synaptic Systems, 234 004 by Susanne Krasemann
Very good antibody that works on human and mouse paraffin embedded tissue sections; no background; very specific. Got one of my favorites!
We performed antigen retrieval in Citrate buffer pH6.0 after deparaffination of the paraffin sections
Dilution 1:500
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