Glutamine synthetase, Synaptic Systems, 367 005

The antibody was used 1:200 (stock concentration 1mg/ml) on mouse dorsal root ganglion tissue for IHC (obs: pretreatment needed – see further down).

Prior to staining the tissue had been fixed in 4% PFA, cryoprotected in 30% sucrose, embedded in TissueTek and cut in 10um sections.

Pretreatment: The slides with tissue sections were baked for 30 min at 60 degrees Celsius. Next, the tissue sections were treated with hydrogen peroxide for 10 min at room temperature. Without the hydrogen peroxide treatment I did not get any positive signal.

The staining looked very specific to the satellite glial cells as expected.