Calbindin, Synaptic Systems, 214 002

Among several antibodies specifically recognizing the calcium-binding protein calbindin-D 28kD, rabbit-anti-calbindin (Synaptic Systems, Göttingen, Germany; 214002) shows excellent staining properties including the visualization of fine processes and other subcellular details even after conventional indirect immunofluorescence labelling. Thereby, it is also useful in combination with guinea pig-anti-parvalbumin (and highly-purified carbocyanine-tagged secondary antibodies detecting either rabbit IgG or guinea pig IgG selectively). The co-expression of both calcium-binding proteins in several neurons of the mouse forebrain after ischemia and in non-affected tissue was recently reported (Härtig et al 2016 Neuroscience – Fig 8). Additionally, the same paper revealed the allocation of calbindin and glutamate decarboxylase (GAD) as the marker enzyme for GABAergic neurons (Fig 9).

I can highly recommend this antibody for IHC

Härtig et al. 2016 Neuroscience: https://www.sciencedirect.com/science/article/abs/pii/S0306452216304420?via%3Dihub