Neurofilament M, Synaptic Systems, 171 204

A very good neurofilament marker is guinea pig-anti-neurofilament, medium (NF-M) from Synaptic Systems in Göttingen (171204). Immunofluorescence labelling with guinea pig-anti-NF-M after experimental stroke in rodents and in human post-mortem stroke tissue was reported by Mages et al 2018 Front Mol Neurosci − Figs 1-3,7,8). For the combined application of this immunoreagent with primary rabbit antibodies, the subsequently secondary antibodies directed against rabbit and guinea pig IgG should be not cross-reacting. (Available highly purified donkey antibodies are for instance from Jackson ImmunoResearch, West Grove, PA, USA; German supplier: Dianova, Hamburg).

Mages et al. 2018 Front Mol Neurosci: https://www.frontiersin.org/articles/10.3389/fncel.2018.00161/full