Iba1, Synaptic Systems, 234 003
What do reviewers generally think about 234 003 from Synaptic Systems?
Researchers consistently report that Iba1, Synaptic Systems, 234 003 performs well for immunohistochemistry of mouse brain tissue, including 4% PFA perfused vibratome sections. One reviewer points out that a 1:1000 dilution with 24 hours at 4°C works well for 30 µm mouse brain sections. It is also described as useful for detecting microglia under physiological conditions in various mammalian species and for identifying microglia/macrophages in ischemic rat and mouse tissue. No complaints are mentioned.
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User Reviews (3)
Review of: Iba1, Synaptic Systems, 234 003 by Shirin Hosseini, Technische Universität Braunschweig
I have used this antibody for immunohistochemistry of 30 µm brain sections of C57BL/6J mice in a dilution of 1:1000. The optimum incubation time for this antibody is 24 hour at 4◦C. This antibody works so well in our lab.
For more information see Hosseini et al. Cell Reports 2020, “Type I Interferon Receptor Signaling in Astrocytes Regulates Hippocampal Synaptic Plasticity and Cognitive Function of the Healthy CNS”.
https://www.sciencedirect.com/science/article/pii/S2211124720306197
0 useful
0 not usefulReview of: Iba1, Synaptic Systems, 234 003 by Suleiman Minhas, Bart De Strooper lab, KU Leuven
We used this antibody for IHC staining of 4% PFA perfused mouse brain tissues (vibratome sections).
It works very well.
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0 useful 0 not usefulReview of: Iba1, Synaptic Systems, 234 003 by Wolfgang Härtig, University of Leipzig
I can highly recommend the following antibody:
Rabbit-anti-Iba (ionized calcium binding adapter molecule 1; Synaptic Systems, Göttingen, Germany; 234003) is useful for the analysis of microglia under physiological conditions in various mammalian species. Moreover, this immunoreagent also enables the detection of microglia/macrophages in ischaemia-affected tissue from rats (Michalski et al 2017 PLoS One – Figs 2, 3 and 13; Bankstahl et al 2018 ENeuro, Figs. 9 and 10) and from mice (Michalski et al 2017 J Alzheimer Dis, Fig 1).
Michalski et al. 2017 PLoS One: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174996
Bankstahl et al. 2018 ENeuro: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5975718/
Michalski et al. 2017 J Alzheimer Dis: https://www.ncbi.nlm.nih.gov/pubmed/28671120
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