CHAT, merckmillipore, AB144P
What do reviewers generally think about AB144P from Sigma-Aldrich?
Researchers report that CHAT, Merck Millipore, AB144P performs well for immunofluorescence and immunohistochemistry in paraformaldehyde-fixed tissue. One reviewer points out that dilutions of 1:600 to 1:1000 worked successfully in floating striatal mouse brain sections, while another used 1:200 for immunohistochemistry. A third reviewer notes expected very good immunofluorescence labeling in PFA-fixed forebrain sections from mouse, hamster, and rat. No complaints are mentioned.
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User Reviews (3)
Review of: CHAT, merckmillipore, AB144P by Robert Lewis
This antibody works really well for immunofluorescence in paraformaldehyde-fixed mouse brain tissue. I have used dilutions from 1:600-1:1000 successfully in floating striatal tissue sections.
Lewis et al 2020: https://www.cell.com/cell-reports/fulltext/S2211-1247(20)30427-7#secsectitle0085
0 useful
0 not usefulReview of: CHAT, merckmillipore, AB144P by Hiromi Kumamaru, University of California
This antibody works fine for immunohistochemistry.
Dilution used: 1:200
For more information see Kumamaru et al. Cell Reports 2019, “Regenerating Corticospinal Axons Innervate Phenotypically Appropriate Neurons within Neural Stem Cell Grafts”
https://www.sciencedirect.com/science/article/pii/S2211124719301366#sec4
Comments
0 useful 0 not usefulReview of: CHAT, merckmillipore, AB144P by Wolfgang Härtig, University of Leipzig
By applying this primary antibody to immunofluorescence labeling you might expect very good results in paraformaldehyde-fixed tissues.
Goat-anti-choline acetyltransferase, affinity-purified) from Merck Millipore (formerly Chemicon; AB144P) applied to forebrain sections from various mammalian species including mouse (Härtig et al 2014, Neuropathol Appl Neurobiol, Fig 2), hamster (Härtig et al 2007 Eur J Neurosci – Figs 4,5 ) and rat (Kacza et al 2000 Brain Res, Fig 3).
Härtig et al. 2014: https://onlinelibrary.wiley.com/doi/full/10.1111/nan.12050
Härtig et al. 2007: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2006.05250.x
Kacza et al. 2000: https://www.sciencedirect.com/science/article/pii/S0006899300022393?via%3Dihub
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0 useful 0 not useful
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