CHAT, merckmillipore, AB144P

This antibody works really well for immunofluorescence in paraformaldehyde-fixed mouse brain tissue. I have used dilutions from 1:600-1:1000 successfully in floating striatal tissue sections.

Lewis et al 2020: https://www.cell.com/cell-reports/fulltext/S2211-1247(20)30427-7#secsectitle0085

This antibody works fine for immunohistochemistry.
Dilution used: 1:200

For more information see Kumamaru et al. Cell Reports 2019, “Regenerating Corticospinal Axons Innervate Phenotypically Appropriate Neurons within Neural Stem Cell Grafts”

https://www.sciencedirect.com/science/article/pii/S2211124719301366#sec4

By applying this primary antibody to immunofluorescence labeling you might expect very good results in paraformaldehyde-fixed tissues.

Goat-anti-choline acetyltransferase, affinity-purified) from Merck Millipore (formerly Chemicon; AB144P) applied to forebrain sections from various mammalian species including mouse (Härtig et al 2014, Neuropathol Appl Neurobiol, Fig 2), hamster (Härtig et al 2007 Eur J Neurosci – Figs 4,5 ) and rat (Kacza et al 2000 Brain Res, Fig 3).

Härtig et al. 2014: https://onlinelibrary.wiley.com/doi/full/10.1111/nan.12050
Härtig et al. 2007: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2006.05250.x
Kacza et al. 2000: https://www.sciencedirect.com/science/article/pii/S0006899300022393?via%3Dihub