GFAP, Synaptic Systems, 173 004
What do reviewers generally think about 173 004 from Synaptic Systems?
Researchers report that GFAP, Synaptic Systems, 173 004 performs well for IHC and immunofluorescence in PFA-fixed brain tissue, including mouse unperfused cryosections and perfused vibratome sections. It is described as usable with mouse, rabbit, and goat secondary antibodies. One reviewer points out that secondary antibodies directed against rabbit and guinea pig IgG should be non-cross-reacting, recommending highly purified donkey antibodies.
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User Reviews (2)
Review of: GFAP, Synaptic Systems, 173 004 by Suleiman Minhas, Bart De Strooper lab, KU Leuven
We used this antibody for IHC staining of mouse brain tissue.
It works well for both un-perfused (cryosections) and 4% PFA perfused (vibratome sections) tissues.
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0 not usefulReview of: GFAP, Synaptic Systems, 173 004 by Wolfgang Härtig, University of Leipzig
By applying this primary antibody to immunofluorescence labeling you might expect very good results in paraformaldehyde-fixed tissues
Guinea pig-anti-glial fibrillary acidic protein (GFAP) from Synaptic Systems (Göttingen, Germany; 173004) – well usable combined with mouse, rabbit and goat antibodies. Importantly, the secondary antibodies directed against rabbit and guinea pig IgG should be not cross-reacting (available highly purified donkey antibodies are for instance from Jackson ImmunoResearch, West Grove, PA, USA; German supplier: Dianova, Hamburg). Some references are Antonucci, Alpár et al 2012, Fig 6;Härtig et al 2014 Neuropathol Appl Neurobiol, Fig 6; Michalski et al 2017 PloS One , Figs 2,3,10-13).
Antonucci et al. 2012: http://www.jneurosci.org/content/32/6/1989.long
Härtig et al. 2014: https://onlinelibrary.wiley.com/doi/full/10.1111/nan.12050
Michalski et al. 2017: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174996
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