NeuN, Synaptic Systems, 266 004
What do reviewers generally think about 266 004 from Synaptic Systems?
Researchers recommend this guinea pig anti-NeuN antibody as a strong IHC marker for neuronal nuclei and perikarya. It works in PFA-fixed brain tissue from rodents, sheep, primates, and even paraffin-embedded alpaca brain, and in mouse brain IHC on unperfused cryosections and 4% PFA perfused vibratome sections. One reviewer points out that indirect immunofluorescence with this antibody visualized neuronal loss after experimental stroke in mice. No performance complaints are mentioned.
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Review of: NeuN, Synaptic Systems, 266 004 by Wolfgang Härtig, University of Leipzig
Searching for a marker detecting a large portion of all neurons, guinea pig-anti-NeuN (neuronal nuclei) from Synaptic Systems (Göttingen, Germany; 266004) can be strongly recommended to visualize the perikarya of nerve cells This antiserum is well applicable investigating paraformaldehyde-fixed brain tissues from various mammalian species, including rodents, sheep and primates. The abilities of this immunoreagent even in paraffin sections from an alpaca’s brain are exemplified by Schöniger et al 2019 (Acta Vet Scand; Fig. 3). Moreover, indirect immunofluorescence labelling of NeuN also visualized neuronal loss after experimental stroke in mice (Härtig et al. 2017, Front Integr Neurosci, Fig. 9).
This antibody is an outstanding immunohistochemical marker
Schöniger et al 2019 Acta Vet Scand: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317207/
Härtig et al. 2017, Front Integr Neurosci: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559442/
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0 not usefulReview of: NeuN, Synaptic Systems, 266 004 by Suleiman Minhas, Bart De Strooper lab, KU Leuven
We used this antibody for IHC staining of mouse brain.
It works well for both un-perfused (cryosections) and 4% PFA perfused (vibratome sections) tissue.
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