Phospho-Tau, Thermo Fischer Scientific, PA1-26693
What do reviewers generally think about PA1-26693 from Thermo Fisher Scientific?
Researchers note the antibody is discontinued and, in a large validation study, it showed poor PTM specificity. It was tested by peptide array, Western blotting of transgenic and knockout mouse brain lysates, and immunofluorescence of human iPSC-derived neurons. One reviewer points out that in the peptide array it detected non-modified Tau peptides as well as Tau phosphorylated at S409, indicating cross-reactivity with unphosphorylated Tau. Overall, the available reviews emphasize lack of specificity for the intended phospho-Tau target.
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Review of: Phospho-Tau, Thermo Fischer Scientific, PA1-26693 by admin
Thermo Fisher has discontinued this antibody
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0 not usefulReview of: Phospho-Tau, Thermo Fischer Scientific, PA1-26693 by Ebru Ercan-Herbst, BioMed X Innovation Center, Heidelberg, Germany
This antibody was included in a study testing 49 commercial available Tau antibodies including both pan-Tau antibodies and antibodies specifically recognizing Tau with post-translational modifications (PTM).
The antibodies were tested by peptide array to assess PTM specificity and by immuno-blotting of mouse brain lysates obtained from hTg-Tau (expressing all six human isoforms of Tau) and Tau knock out mice to test Tau specificity. A subset of 35 antibodies was subsequently tested by immunofluorescence staining of human iPSC derived neurons (see reference below for further details)
The study was published in Ercan et al. Molecular Neurodegeneration (2017) 12:87 “A validated antibody panel for the characterization of tau post-translational modifications” and demonstrates that commercially available antibodies show a significant lack of specificity. In particular, PTM-specific antibodies very often recognize non-modified version of Tau.
In the peptide array this antibody detected non-modified Tau peptides as well as Tau peptides phosphorylated at S409
(https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0229-1)
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