Phospho-Tau, Thermo Fischer Scientific, PA5-35762
What do reviewers generally think about PA5-35762 from Thermo Fisher Scientific?
Researchers provide limited but positive performance feedback, noting the antibody was evaluated in a validation study of Tau antibodies using peptide arrays, Western blotting, and immunofluorescence. One reviewer points out that it has been discontinued and replaced by Invitrogen Catalog # 44-742G. Another reports very good Western blot performance at 1/500, with strong Tau signal and no non-specific signal in mouse brain lysates, and notes its use in testing PTM specificity and Tau specificity.
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User Reviews (2)
Review of: Phospho-Tau, Thermo Fischer Scientific, PA5-35762 by admin
This antibody has been discontinued and replaced with Invitrogen Catalog # 44-742G
0 useful
0 not usefulReview of: Phospho-Tau, Thermo Fischer Scientific, PA5-35762 by Ebru Ercan-Herbst, BioMed X Innovation Center, Heidelberg, Germany
This antibody was included in a study testing 49 commercial available Tau antibodies including both pan-Tau antibodies and antibodies specifically recognizing Tau with post-translational modifications (PTM).
The antibodies were tested by peptide array to assess PTM specificity and by immuno-blotting of mouse brain lysates obtained from hTg-Tau (expressing all six human isoforms of Tau) and Tau knock out mice to test Tau specificity. A subset of 35 antibodies was subsequently tested by immunofluorescence staining of human iPSC derived neurons (see reference below for further details)
WB: 1/500, Very good – strong signal for tau, no non-specific signal
The study was published in Ercan et al. Molecular Neurodegeneration (2017) 12:87 “A validated antibody panel for the characterization of tau post-translational modifications” and demonstrates that commercially available antibodies show a significant lack of specificity. In particular, PTM-specific antibodies very often recognize non-modified version of Tau.
(https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0229-1)
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