Tau-4R, merckmillipore, 05-804
What do reviewers generally think about 05-804 from Sigma-Aldrich?
Researchers report mixed performance for Tau-4R in IHC on PFA mouse brain, with batch inconsistency: the first batch worked okay, while later batches gave poor results. In a validation study, it showed strong tau signal and no nonspecific signal by Western blot at 1/1000 and was tested by peptide arrays, mouse brain lysates, and iPSC neuron immunofluorescence. One reviewer points out that the study found many commercial tau antibodies, particularly PTM-specific ones, often recognize non-modified tau.
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Review of: Tau-4R, merckmillipore, 05-804 by Suleiman Minhas, Bart De Strooper lab, KU Leuven
We have used this antibody for IHC staining of 4% PFA perfused mouse brain tissue (vibratome sections). The first batch of the antibody worked okay, but the second and third batch didn’t give good results.
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0 not usefulReview of: Tau-4R, merckmillipore, 05-804 by Ebru Ercan-Herbst, BioMed X Innovation Center, Heidelberg, Germany
This antibody was included in a study testing 49 commercial available Tau antibodies including both pan-Tau antibodies and antibodies specifically recognizing Tau with post-translational modifications (PTM).
The antibodies were tested by peptide array to assess PTM specificity and by immuno-blotting of mouse brain lysates obtained from hTg-Tau (expressing all six human isoforms of Tau) and Tau knock out mice to test Tau specificity. A subset of 35 antibodies was subsequently tested by immunofluorescence staining of human iPSC derived neurons (see reference below for further details)
WB: 1/1000, Very good – strong signal for tau, no non-specific signal
The study was published in Ercan et al. Molecular Neurodegeneration (2017) 12:87 “A validated antibody panel for the characterization of tau post-translational modifications” and demonstrates that commercially available antibodies show a significant lack of specificity. In particular, PTM-specific antibodies very often recognize non-modified version of Tau.
(https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0229-1)
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