Tau, Agilent Dako, A0024

By applying this primary antibody to immunofluorescence labeling you might expect very good results in paraformaldehyde-fixed tissues.

Rabbit-anti-(microtubule-associated protein) tau from Dakocytomation (Glostrup, Denmark, A0024; German provider: Dakocytomation, Hamburg): well usable in an experimental mouse model for stroke (Michalski et al 2016, Figs 1-5; Härtig et al 2016 – Figs 1,6,7).

Michalski et al 2016: https://www.sciencedirect.com/science/article/pii/S0306452216301622?via%3Dihub

Härtig et al 2016: https://www.sciencedirect.com/science/article/pii/S0306452216304420?via%3Dihub

This antibody was included in a study testing 49 commercial available Tau antibodies including both pan-Tau antibodies and antibodies specifically recognizing Tau with post-translational modifications (PTM).

The antibodies were tested by peptide array to assess PTM specificity and by immuno-blotting of mouse brain lysates obtained from hTg-Tau (expressing all six human isoforms of Tau) and Tau knock out mice to test Tau specificity. A subset of 35 antibodies was subsequently tested by immunofluorescence staining of human iPSC derived neurons (see reference below for further details)

WB: 1/2500, Good – strong signal for tau, weak non-specific signal

The study was published in Ercan et al. Molecular Neurodegeneration (2017) 12:87 “A validated antibody panel for the characterization of tau post-translational modifications” and demonstrates that commercially available antibodies show a significant lack of specificity. In particular, PTM-specific antibodies very often recognize non-modified version of Tau.

(https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0229-1)