CD4, BD Bioscience, 562970
What do reviewers generally think about 562970 from BD Bioscience?
Researchers report the BV510-conjugated CD4 antibody performs well in multicolor flow cytometry for phenotyping PBMCs, expanded CAR T cells, and lymphocytes in activation panels. It gives good CD4/CD8 separation and works with CD8 and CD137. One reviewer points out that 1 uL, titrated on T cells, stained 200-300,000 cells in about 100 uL PBS plus 2% FCS. Another used 2 uL at 4 degrees.
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Review of: CD4, BD Bioscience, 562970 by Kasper Mølgaard Jensen
Antibody used in a multicolor flow cytometry panel to phenotype PBMCs and expanded CAR T cells. Works very well in separation with CD8 (BD Horizon #566860). 200-300.000 PBMCs or T cells were washed once in PBS and stained in approximately 100 uL PBS + 2 % FCS using 1 uL antibody (antibody has been titrated on T cells).
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0 not usefulReview of: CD4, BD Bioscience, 562970 by Arianna Draghi, Center for Cancer Immune Therapy, Herlev Hospital
CD4 (human), mouse mAb (clone SK3), BV510 conjugated, BD Biosciences, 562970
Used to stain lymphocytes in an activation panel along with (CD8 and CD137).
2uL used to stain cells in a FACS tube at 4 degrees.
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