CD107a, BioLegend, 328626
What do reviewers generally think about 328626 from BioLegend?
Researchers used this antibody for intracellular CD107a staining in ICS assays, adding it to a Golgi Plug mastermix in 96-well plates during 37°C incubation. Both note that titration and compensation were performed in-lab. One reviewer points out that 0.3 µL was mixed into the mastermix for the full 5-7 hour incubation, while another specifies 0.3 µL in 20 µL mastermix for about 300,000 cells. The reviews do not mention performance complaints.
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User Reviews (2)
Review of: CD107a, BioLegend, 328626 by Arianna Draghi
Used to stain cells for the intracellular expression of CD107a in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in 96-well plate during assay incubation in combination with a protein transport inhibitor (Golgi Plug). Antibody titrations and compensation were previously performed in-lab.
0,3uL mixed in a GolgiPlud mastermix. mastermix added to each well in a 96-well plate for the duration of assay incubation at 37C (5-7h)
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0 not usefulReview of: CD107a, BioLegend, 328626 by Kasper Mølgaard Jensen
Used to stain cells for the intracellular expression of CD107a in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in 96-well plate during assay incubation in combination with a protein transport inhibitor (Golgi Plug). Antibody titrations and compensation were previously performed in-lab.
Working solution of 0,3uL in 20uL mastermix to stain approx. 300.000 cells.
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