TNF, BD Bioscience, 554541
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User Reviews (2)
Review of: TNF, BD Bioscience, 554541 by Arianna Draghi
Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
a total of 1,0uL used in a 20uL mastermix with anti-IFN used to stain approx. 300.000 cells.
Review of: TNF, BD Bioscience, 554541 by Kasper Mølgaard Jensen
Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
Working solution of 1uL in 20uL mastermix to stain approx. 300.000 cells.
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