TNF, BD Bioscience, 554541
What do reviewers generally think about 554541 from BD Bioscience?
Researchers used this antibody to stain cells for intracellular cytokine expression in ICS assays, including TNF, IFNγ, and CD107a, by mixing it into a mastermix after assay incubation and permeabilization for FACS. Both reviews state that titrations and compensation had been performed in-lab. One reviewer points out that a total of 1.0 uL in a 20 uL mastermix with anti-IFN was used to stain approximately 300,000 cells. No specific staining-quality complaints are mentioned.
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User Reviews (2)
Review of: TNF, BD Bioscience, 554541 by Arianna Draghi
Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
a total of 1,0uL used in a 20uL mastermix with anti-IFN used to stain approx. 300.000 cells.
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0 not usefulReview of: TNF, BD Bioscience, 554541 by Kasper Mølgaard Jensen
Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
Working solution of 1uL in 20uL mastermix to stain approx. 300.000 cells.
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