CD56, BD Bioscience, 345812
What do reviewers generally think about 345812 from BD Bioscience?
Both reviewers used CD56, BD Bioscience, 345812 for phenotype gating in an intracellular cytokine secretion assay measuring TNF, IFNγ, and CD107a. They added it to a mastermix with CD3, CD4, and CD8 after assay incubation and before fixation and permeabilization, using 3 uL in 20 uL for about 300,000 cells. One reviewer points out that antibody titrations and compensation had been performed in-lab beforehand. The reviews do not report staining quality, brightness, or complaints.
Information about this antibody
Vendor notes
User Reviews (2)
Review of: CD56, BD Bioscience, 345812 by Arianna Draghi
Used to stain cells for phenotype gating in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and prior to fixation and permeabilization (CD3, CD4, CD8, CD56). Antibody titrations and compensation were previously performed in-lab.
a total of 3,0uL used in a 20uL mastermix with CD3, CD4 and CD8 used to stain approx. 300.000 cells.
0 useful
0 not usefulReview of: CD56, BD Bioscience, 345812 by Kasper Mølgaard Jensen
Used to stain cells for phenotype gating in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and prior to fixation and permeabilization (CD3, CD4, CD8, CD56). Antibody titrations and compensation were previously performed in-lab.
Working solution of 3uL in 20uL mastermix to stain approx. 300.000 cells.
Comments
0 useful 0 not useful
Comments