α2A-AR, Sigma-Aldrich, sab4500548

In “Commercially available antibodies directed against α-adrenergic receptor subtypes and other G protein-coupled receptors with acceptable selectivity in flow cytometry experiments”, Tripathi et al study the specificity of 8 different antibodies against α-adrenergic receptor (AR) subtypes. The specificity of the antibodies was tested by flow cytometry using human vascular smooth muscle cell. Here, a fluorescent signal was measured when treated with siRNA specific for the α-adrenergic receptor (Human ADRA2A (150) (E-005422-00-0050))) and compared with the fluorescent signal from cells treated with non-targeting RNA.

When using this antibody, a 46% reduction in fluorescence was observed.

See full description in the article:
https://www.ncbi.nlm.nih.gov/pubmed/26660071