M2 Muscarinic Receptor, Chemicon, AB5166
Information about this antibody
Vendor notes
User Reviews (1)
Review of: M2 Muscarinic Receptor, Chemicon, AB5166 by Camilla Gustafsen, Aarhus Universitet
Jositsch and colleagues from Justus-Liebig-University Giessen reported in 2009 an extensive study, in which they evaluate 24 muscarinic acetylcholine receptor antibodies including 16 commercially available.
Antibodies directed against the five identified subtypes of muscarinic receptor (M1R-M5R) were tested in immunohistochemical labelling of wild type mouse tissue, and staining was compared to labelling of control tissue obtained from the following MR gene-deficient (-/-) mice: M1R-/-, M2R-/-, M3R-/-, M4R-/-, M5R-/- and M2/3R-/-.
The researchers found that some batches of this antibody produced a specific labelling of WT tissue, which was not observed in M2R knockout tissue. However, other batches of the antibody produced identical staining of WT and control samples.
For more information see:
Jositsch et al. 2009, Suitability of muscarinic acetylcholine receptor antibodies for immunohistochemistry evaluated on tissue sections of receptor gene-deficient mice
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3896859/
0 useful
0 not useful
Comments